Title of Dissertation: DELIVERY OF DNA AND RECOMBINANT INFECTIOUS BURSAL DISEASE VIRUS VACCINES IN OVO
نویسندگان
چکیده
In ovo vaccination against Marek’s disease virus (MDV) is a common practice in more than 85% of broilers produced in the US. DNA vaccines represent a new tool to prevent infectious diseases in many species, including poultry. An in ovo delivery system for plasmid DNA vaccines is described in which we evaluate the route of delivery (air cell vs amniotic cavity), transfection reagent (IFA+DMSO vs polyethylenimine), dose of plasmid DNA (1 to 100 μg/egg), and the nature of humoral immune responses. A plasmid DNA (CMV-EGFP-BGH) construct expressing enhanced green fluorescent protein (EGFP) under cytomegalovirus (CMV) immediate early promoter was used to optimize the route of delivery, and formulation for in ovo DNA vaccination. A plasmid expressing the hemmagglutinin-neuraminidase (HN) gene of Newcastle disease virus (pIRES-HN-EGFP) was used to evaluate five different dosages of DNA and the humoral immune responses after in ovo vaccination.
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